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Staphylococcus

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Staphylococcus.

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Staphylococcus

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To find out if testing of up to 10 Staphylococcus aureus isolates from each sample from raw milk and raw milk products for staphylococcal enterotoxin (SE) might increase the chances of identifying potential sources of food intoxication.Methods and Results:  Altogether 386 S. aureus isolates were tested for the presence of SE by reversed passive latex agglutination (SET-RPLA), and SE genes (se) by a multiplex polymerase chain reaction (PCR). In 18 of 34 (53%) S. aureus positive samples a mixture of SE and/or se positive and negative isolates were identified. Multiplex PCR increased the number of potential SE producing strains, i.e. isolates that harboured se, with 51% among the product and 48% among the raw bovine milk isolates. Examination by pulsed-field gel electrophoresis mostly confirmed clonal similarity among isolates sharing SE/se profile, but did not further differentiate between them.Conclusions:  Isolates of S. aureus collected from one sample may show great diversity in SE production and different plating media seem to suppress or favour different strains of S. aureus.Significance and Impact of the Study:  Several isolates of S. aureus from each sample should be tested for enterotoxin production in cases with typical SE intoxication symptoms with methods that are able to reveal new SE/se.

Staphylococcus aureus.

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Methicillin-resistant Staphylococcus aureus is an important cause of skin and soft tissues infections but also of more dangerous infections (infective endocarditis, for example).
At the beginning, this type of infection is developed in hospital. Nowadays, MRSA can also be found in the extra-hospital community.
Their scattering is in increase but the effective treatments are in decrease. As MRSA became more resistant to numerous antibiotics. A lot of c1inical studies are realized in an aim of supplying the most effective treatment and of avoiding new resistances.
Amongst these studies, many conclusions lead to insist on prevention and screening.
These prevention measures as well as the use of various antibiotics having an action on the MRSA will be developed in this thesis. Le staphylococcus aureus résistant à la méthicilline (MRSA) est une cause importante d'infections de la peau et des tissus mous mais aussi d'infections plus graves (endocardite, par exemple).
Au départ, ce type d'infections étaient acquis uniquement à l'hôpital. Aujourd'hui, les MRSA se retrouvent aussi dans la communauté.
Leur dissémination est en augmentation mais les traitements efficaces diminuent. En effet, les MRSA sont devenus co-résistants à de nombreux antibiotiques.
Beaucoup d'études cliniques sont réalisées dans le but de fournir le traitement le plus efficace possible et d'éviter la sélection de nouvelles résistances. Parmi ces études, beaucoup insistent sur la prévention et le dépistage.
Ces mesures de prévention ainsi que les différents antibiotiques ayant une action sur les MRSA seront développés dans ce mémoire.

Staphylococcus aureus --- Methicillin-Resistant Staphylococcus aureus --- Staphylococcus --- Drug Resistance